Coding
Part:BBa_K2749017:Design
Designed by: iGEM 2018 Ruia-Mumbai Team Group: iGEM18_Ruia-Mumbai (2018-10-10)
xylE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 669
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is codon optimized for chassis E.coli and ensured to have no EcoR1 and Pst1 restriction sites in-between the sequence.
Source
xylE is derived from Pseudomonas putida